Highlights
- •Endosequence Root Repair Material has a similar antifungal activity compared with mineral trioxide aggregate.
- •Endosequence Root Repair Material's initial set will have more antifungal activity.
- •Endosequence Root Repair Material and mineral trioxide aggregate can change the environment (pH) to exert antifungal activity.
Abstract
Introduction
The purpose of this study was to investigate the antifungal activity of Endosequence
Root Repair Material (ERRM; Brasseler USA, Savannah, GA) as compared with mineral
trioxide aggregate (MTA) using Candida albicans.
Methods
All materials were packed into sterilized intravenous tubing to obtain standardized
samples and allowed to set for 3 or 24 hours and then exposed to a suspension of C. albicans for incubations of 24 or 48 hours. To analyze the mechanisms of the material's antifungal
activity, additional samples of each test material were prepared in the same manner
and allowed to set for 24 hours; these were then incubated in a culture medium for
24 hours. The pH of each conditioned media was measured before transferring to wells
containing C. albicans. The development of biofilm was analyzed after 24 and 48 hours with 2,3-bis (2-methoxy-4-nitro-5-sulfophenyl)-5-([phenyl
amino] carbonyl)-2H-tetrazolium hydroxide reduction assay.
Results
Materials in both experimental groups significantly limited biofilm formation at each
interval (ie, 24 and 48 hours). After incubating for a 24-hour period in the presence
of C. albicans, ERRM in both experimental groups showed a reduction in biofilm formation that was
statistically significant in comparison with MTA. However, when set for 24 hours and
incubated for 48 hours, gray MTA and white MTA showed a more substantial reduction
in biofilm formation than comparable samples of ERRM. Cultured media conditioned with
test materials showed statistically significant antifungal biofilm activity after
48 hours.
Conclusions
All materials tested have comparable antifungal biofilm activity. It appeared that
changing the environment, such as the pH, contributed to this activity.
Key Words
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Article info
Publication history
Published online: September 13, 2014
Identification
Copyright
© 2014 American Association of Endodontists. Published by Elsevier Inc. All rights reserved.
