- •Endosequence Root Repair Material has a similar antifungal activity compared with mineral trioxide aggregate.
- •Endosequence Root Repair Material's initial set will have more antifungal activity.
- •Endosequence Root Repair Material and mineral trioxide aggregate can change the environment (pH) to exert antifungal activity.
The purpose of this study was to investigate the antifungal activity of Endosequence Root Repair Material (ERRM; Brasseler USA, Savannah, GA) as compared with mineral trioxide aggregate (MTA) using Candida albicans.
All materials were packed into sterilized intravenous tubing to obtain standardized samples and allowed to set for 3 or 24 hours and then exposed to a suspension of C. albicans for incubations of 24 or 48 hours. To analyze the mechanisms of the material's antifungal activity, additional samples of each test material were prepared in the same manner and allowed to set for 24 hours; these were then incubated in a culture medium for 24 hours. The pH of each conditioned media was measured before transferring to wells containing C. albicans. The development of biofilm was analyzed after 24 and 48 hours with 2,3-bis (2-methoxy-4-nitro-5-sulfophenyl)-5-([phenyl amino] carbonyl)-2H-tetrazolium hydroxide reduction assay.
Materials in both experimental groups significantly limited biofilm formation at each interval (ie, 24 and 48 hours). After incubating for a 24-hour period in the presence of C. albicans, ERRM in both experimental groups showed a reduction in biofilm formation that was statistically significant in comparison with MTA. However, when set for 24 hours and incubated for 48 hours, gray MTA and white MTA showed a more substantial reduction in biofilm formation than comparable samples of ERRM. Cultured media conditioned with test materials showed statistically significant antifungal biofilm activity after 48 hours.
All materials tested have comparable antifungal biofilm activity. It appeared that changing the environment, such as the pH, contributed to this activity.
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- Mineral trioxide aggregate material use in endodontic treatment: a review of the literature.Dent Mater. 2008; 24: 149-164
- Prognostic factors in apical surgery with root-end filling: a meta-analysis.J Endod. 2010; 36: 957-973
- Clinical results with two different methods of root-end preparation and filling in apical surgery: mineral trioxide aggregate and adhesive resin composite.J Endod. 2010; 36: 1122-1129
- Outcome of endodontic surgery: a meta-analysis of the literature–part 1: comparison of traditional root-end surgery and endodontic microsurgery.J Endod. 2010; 36: 1757-1765
- Mineral trioxide aggregate: a comprehensive literature review—part I: chemical, physical, and antibacterial properties.J Endod. 2010; 36: 16-27
- Cytotoxicity evaluation of endosequence root repair material.Oral Surg Oral Med Oral Pathol Oral Radiol Endod. 2010; 109: e122-e125
- A comparison of the cytotoxicity and proinflammatory cytokine production of EndoSequence root repair material and ProRoot mineral trioxide aggregate in human osteoblast cell culture using reverse-transcriptase polymerase chain reaction.J Endod. 2012; 38: 486-489
- An evaluation of setting time of mineral trioxide aggregate and EndoSequence root repair material in the presence of human blood and minimal essential media.J Endod. 2013; 39: 1071-1072
- Pathogenesis of apical periodontitis and the causes of endodontic failures.Crit Rev Oral Biol Med. 2004; 15: 348-381
- Biofilms and apical periodontitis: study of prevalence and association with clinical and histopathologic findings.J Endod. 2010; 36: 1277-1288
- Intraradicular bacteria and fungi in root-filled, asymptomatic human teeth with therapy-resistant periapical lesions: a long-term light and electron microscopic follow-up study.J Endod. 1990; 16: 580-588
- Observation of bacteria and fungi in infected root canals and dentinal tubules by SEM.Endod Dent Traumatol. 1995; 11: 6-9
- Fungi in therapy-resistant apical periodontitis.Int Endod J. 1997; 30: 96-101
- Occurrence of Candida albicans in infections of endodontic origin.J Endod. 2000; 26: 695-698
- Predictable management of cracked teeth with reversible pulpitis.Aust Dent J. 2009; 54: 306-315
- Acute pulpal-alveolar cellulitis syndrome. III. Endodontic therapeutic factors and the resolution of a Candida albicans infection.Oral Surg Oral Med Oral Pathol. 1981; 52: 630-634
- Yeasts in apical periodontitis.Crit Rev Oral Biol Med. 2003; 14: 128-137
- Fungi in endodontic infections.Oral Surg Oral Med Oral Pathol Oral Radiol Endod. 2004; 97: 632-641
- Microorganisms in root canal-treated teeth from a German population.J Endod. 2008; 34: 926-931
- Diversity and frequency of yeasts from the dorsum of the tongue and necrotic root canals associated with primary apical periodontitis.Int Endod J. 2009; 42: 839-844
- Effect of white-colored mineral trioxide aggregate in different concentrations on Candida albicans in vitro.J Endod. 2005; 31: 684-686
- Comparison of antifungal activity of white-colored and gray-colored mineral trioxide aggregate (MTA) at similar concentrations against Candida albicans.J Endod. 2006; 32: 365-367
- Evaluation of antifungal activity of mineral trioxide aggregate.J Endod. 2003; 29: 826-827
- Isolation and taxonomy of filamentous fungi in endodontic infections.J Endod. 2010; 36: 626-629
- Biofilm formation by the fungal pathogen Candida albicans: development, architecture, and drug resistance.J Bacteriol. 2001; 183: 5385-5394
- Our current understanding of fungal biofilms.Critical reviews in microbiology. 2009; 35: 340-355
- Influence of a bioceramic root end material and mineral trioxide aggregates on fibroblasts and osteoblasts.Arch Oral Biol. 2013; 58: 1232-1237
- Antibacterial activity of endosequence root repair material and proroot MTA against clinical isolates of Enterococcus faecalis.J Endod. 2011; 37: 1542-1546
- Mineral trioxide aggregate (MTA) solubility and porosity with different water-to-powder ratios.J Endod. 2003; 29: 814-817
- Low temperature degradation -aging- of zirconia: a critical review of the relevant aspects in dentistry.Dent Mater. 2010; 26: 807-820
- Bacterial colonization of zirconia ceramic surfaces: an in vitro and in vivo study.Int J Oral Maxillofac Implants. 2002; 17: 793-798
- A specific inhibitor of keratinolytic proteinase from Candida albicans could inhibit the cell growth of C. albicans.J Invest Dermatol. 1985; 85: 438-440
- pH changes in dental tissues after root canal filling with calcium hydroxide.J Endod. 1981; 7: 17-21
- Effect of calcium hydroxide treatment on periodontal repair and root resorption.Endod Dent Traumatol. 1986; 2: 184-189
- Comparison of intracanal EndoSequence Root Repair Material and ProRoot MTA to induce pH changes in simulated root resorption defects over 4 weeks in matched pairs of human teeth.J Endod. 2011; 37: 502-506
Published online: September 13, 2014
© 2014 American Association of Endodontists. Published by Elsevier Inc. All rights reserved.