Highlights
- •The long-term antibacterial effectiveness of sodium hypochlorite (NaOCl) followed by different root canal sealers against Enterococcus faecalis biofilms in dentin is evaluated using a dentin infection model.
- •The proportion of killed bacteria increases significantly during the first 30 days of exposure to the sealers but remains stable after that.
- •Significantly more bacteria are dead when NaOCl and sealers (exposure for 30 and 60 days) are used in combination than when used alone.
- •Specimens with MTA Fillapex after 5% NaOCl exhibit the highest percentage of bacterial killing.
Abstract
Introduction
The present study aimed to evaluate the antibacterial effect of the combined use of
sodium hypochlorite (NaOCl) and root canal sealers on Enterococcus faecalis biofilms using a dentin infection model.
Methods
Cells of E. faecalis were introduced into the dentinal tubules by centrifugation and incubated in brain-heart
infusion for 3 weeks. The biofilms in dentin were first subjected to 5% NaOCl or sterile
water for 10 minutes followed by an equal thickness of AH Plus (Dentsply International
Inc, York, PA), Endosequence BC Sealer (Brasseler USA, Savannah, GA), or MTA Fillapex
(Angelus Indústria de Produtos Odontológicos S/A, Londrina, Brazil) placed on the
root canal wall of the dentin specimens for 7, 30, and 60 days. Gutta-percha and water
were used in a similar manner as controls. The proportions of dead and live bacteria
inside the dentinal tubules were assessed by confocal laser scanning microscopy and
viability staining.
Results
The combined use of NaOCl and sealers (30 and 60 days) killed significantly more bacteria
than NaOCl or sealers alone (P < .05). NaOCl + MTA Fillapex was the most effective antibacterial combination by
killing 83% bacteria in dentin tubules in 60 days. Thirty and 60 days of exposure
to the sealers resulted in significantly more dead bacteria in dentin biofilms than
7-day exposures (P < .05).
Conclusions
The placement of root canal sealer after NaOCl treatment enhanced antibacterial effects
against E. faecalis in the dentinal tubules. Little additional effect was obtained after 30 days of exposure
to sealers.
Key Words
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Article info
Publication history
Published online: June 16, 2015
Identification
Copyright
© 2015 American Association of Endodontists. Published by Elsevier Inc. All rights reserved.
