Highlights
- •Our results show that the previously less explored complement C5a receptor C5L2 constitutes a negative regulator of the neurite outgrowth under lipoteichoic acid stimulation.
- •Of the events occurring during dentin-pulp regeneration, nerve regeneration is the key factor for maintaining tooth viability after infection or injury.
- •A new mechanism of the complement system and the novel receptor identified in this study may provide a useful future therapeutic tool in targeting the pulp fibroblasts in the dentin-pulp regeneration process.
Abstract
Introduction
We recently reported that caries-associated C5a receptor (C5aR) expression and activation
result in up-regulation of brain-derived neurotropic factor secretion by pulp fibroblasts
inducing prominent neurite outgrowth toward the carious site. Our data further showed
a negative regulation of this brain-derived neurotropic factor secretion by C5L2,
another C5aR. C5L2 was considered a nonfunctional receptor and thus has received much
less attention than C5aR. The aim of this study was to identify the role of C5L2 in
pulp fibroblast–mediated neurite outgrowth.
Methods
In this study, lipoteichoic acid (LTA) was used to mimic dental caries–like inflammation.
To evaluate the role of C5L2 in pulp neurite outgrowth, human pulp fibroblasts were
C5L2 small interfering RNA silenced and cocultured with human neurons in a nerve growth
assay system.
Results
C5L2 silencing drastically increased the neurite outgrowth toward the LTA-stimulated
pulp fibroblasts. The number of neurites detected was increased in the LTA-treated
pulp fibroblasts.
Conclusions
Our results show that C5L2 constitutes a negative regulator of the neurite outgrowth
under LTA stimulation. Of the events occurring during dentin-pulp regeneration, nerve
regeneration is the key factor for maintaining tooth viability after infection or
injury. Our study provides a foundation for creating therapeutic tools that target
pulp fibroblasts during pulp/nerve regeneration.
Key Words
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Article info
Publication history
Published online: July 19, 2018
Identification
Copyright
© 2018 American Association of Endodontists.
