Abstract
Introduction
This ex vivo study compared the disinfection and shaping abilities of 2 preparation protocols
in C-shaped canals.
Methods
Mandibular second molars with type I C-shaped canals were pair matched based on micro–computed
tomographic (micro-CT) analysis and distributed into 2 groups. The canals were contaminated
with a mixed bacterial culture and prepared using 2 protocols, both with 2.5% sodium
hypochlorite irrigation: XP-endo Shaper (FKG Dentaire, La Chaux-de-Fonds, Switzerland)
supplemented with XP-endo Finisher (FKG Dentaire) (XP-E) and BioRaCe (FKG Dentaire)
supplemented with a Hedström file (Dentsply/Sirona, Tulsa, OK) (BR-Hed). Micro-CT
scans and intracanal bacteriologic samples were taken before (scan #1 and sample 1)
and after preparation (scan #2 and sample 2) and after the supplementary step (scan
#3 and sample 3). Canal shaping was evaluated by micro-CT imaging, and disinfection
was assessed by quantitative polymerase chain reaction.
Results
Micro-CT data from the XP-E (n = 21) and BR-Hed (n = 23) protocols revealed no significant differences between groups regarding shaping
parameters (canal volume, surface area, structure model index, and prepared walls)
after preparation and after the supplementary step (P > .05). All these parameters significantly increased after each preparation step
(P < .05), except for structure model index changes after the XP-endo Finisher (P > .05). Bacteriologic data from the XP-E (n = 21) and BR-Hed (n = 22) groups showed that 14 (66.7%) and 10 (45.5%) S2 samples still had detectable
bacteria, respectively. The corresponding figures for supplementary steps with the
XP-endo Finisher and Hedström file were 11 (52.4%) and 10 (45.5%), respectively. Bacterial
counts in sample 1 significantly decreased in samples 2 and 3 in both groups (P < .01). Intragroup comparison between sample 2 and sample 3 showed a 66% reduction
in counts after using the Hedström file and 18% after using the XP-endo Finisher (P > .05 for both groups). The quantitative bacterial reduction was not different between
groups (P > .05).
Conclusions
The XP-endo Shaper and BioRaCe systems have similar disinfecting and shaping abilities
in C-shaped canals. Supplementary steps with the Hedström file and the XP-endo Finisher
were similarly effective in improving shaping, but this was not sufficient to enhance
disinfection. About half of the cases, in both groups, still harbored detectable amounts
of bacteria.
Key Words
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References
- What happens to unprepared root canal walls: a correlative analysis using micro-computed tomography and histology/scanning electron microscopy.Int Endod J. 2018; 51: 501-508
- Endodontic therapy in teeth with anatomical variations.in: Rotstein I. Ingle J.I. Ingle’s Endodontics. 7th ed. PMPH USA, Raleigh, NC2019: 866-869
- C-shaped canal system in mandibular second molars: part I--anatomical features.J Endod. 2004; 30: 899-903
- Micro-computed tomographic analysis of mandibular second molars with C-shaped root canals.J Endod. 2015; 41: 890-895
- Anatomical study of C-shaped canals in mandibular second molars by analysis of computed tomography.J Endod. 2006; 32: 10-13
- C-shaped root canal system in mandibular second molars in a Chinese population evaluated by cone-beam computed tomography.Int Endod J. 2011; 44: 857-862
- Worldwide prevalence of mandibular second molar C-shaped morphologies evaluated by cone-beam computed tomography.J Endod. 2017; 43: 1442-1447
- Micro-computed tomographic comparison of nickel-titanium rotary versus traditional instruments in C-shaped root canal system.J Endod. 2010; 36: 708-712
- Effect of finishing instrumentation using NiTi hand files on volume, surface area and uninstrumented surfaces in C-shaped root canal systems.Int Endod J. 2017; 50: 604-611
- The challenge of C-shaped canal systems: a comparative study of the self-adjusting file and ProTaper.J Endod. 2012; 38: 209-214
- Cleaning, shaping, and disinfecting abilities of 2 instrument systems as evaluated by a correlative micro-computed tomographic and histobacteriologic approach.J Endod. 2020; 46: 846-857
- Adjunctive steps for disinfection of the mandibular molar root canal system: a correlative bacteriologic, micro-computed tomography, and cryopulverization approach.J Endod. 2016; 42: 1667-1672
- Evaluation of several instrumentation techniques and irrigation methods on the percentage of untouched canal wall and accumulated dentine debris in C-shaped canals.Int Endod J. 2019; 52: 1354-1365
- Mechanical reduction in intracanal Enterococcus faecalis when using three different single-file systems: an ex vivo comparative study.Int Endod J. 2019; 52: 77-85
- Bacteria and hard tissue debris extrusion and intracanal bacterial reduction promoted by XP-endo Shaper and Reciproc instruments.J Endod. 2018; 44: 1173-1178
- Distribution of sodium hypochlorite throughout the mesial root canal system of mandibular molars after adjunctive irrigant activation procedures: a micro-computed tomographic study.Clin Oral Investig. 2020; 24: 907-914
- Efficacy of 4 irrigation protocols in killing bacteria colonized in dentinal tubules examined by a novel confocal laser scanning microscope analysis.J Endod. 2016; 42: 928-934
- In vitro efficacy of XP-endo Finisher with 2 different protocols on biofilm removal from apical root canals.J Endod. 2017; 43: 321-325
- Evaluation of a rotary instrumentation method for C-shaped canals with micro-computed tomography.J Endod. 2008; 34: 1233-1238
- C-shaped canal system in mandibular second molars part IV: 3-D morphological analysis and transverse measurement.J Endod. 2006; 32: 1062-1065
- Micro-CT evaluation of the efficacy of hard-tissue removal from the root canal and isthmus area by positive and negative pressure irrigation systems.Int Endod J. 2016; 49: 1079-1087
- Quantitative microbiological study of human carious dentine by culture and real-time PCR: association of anaerobes with histopathological changes in chronic pulpitis.J Clin Microbiol. 2002; 40: 1698-1704
- Root canal disinfection by single- and multiple-instrument systems: effects of sodium hypochlorite volume, concentration, and retention time.J Endod. 2019; 45: 736-741
- Clinical efficacy of treatment procedures in endodontic infection control and one year follow-up of periapical healing.J Endod. 2005; 31: 863-866
- Outcome of endodontic retreatment using 2 root canal irrigants and influence of infection on healing as determined by a molecular method: a randomized clinical trial.J Endod. 2019; 45: 1089-1098.e5
- Influence of the apical preparation size and the irrigant type on bacterial reduction in root canal-treated teeth with apical periodontitis.J Endod. 2017; 43: 1058-1063
- Supplementing the antimicrobial effects of chemomechanical debridement with either passive ultrasonic irrigation or a final rinse with chlorhexidine: a clinical study.J Endod. 2012; 38: 1202-1206
- Antibacterial effectiveness of 2 root canal irrigants in root-filled teeth with infection: a randomized clinical trial.J Endod. 2016; 42: 1307-1313
- Disinfecting oval-shaped root canals: effectiveness of different supplementary approaches.J Endod. 2011; 37: 496-501
- The effects of different endodontic access cavity design and using xp-endo finisher on the reduction of Enterococcus faecalis in the root canal system.J Endod. 2020; 46: 419-424
- Effects of contemporary irrigant activation schemes and subsequent placement of an interim dressing on bacterial presence and activity in root canals associated with asymptomatic apical periodontitis.J Clin Med. 2020; 9: 854
- Exploiting molecular methods to explore endodontic infections: Part 1--current molecular technologies for microbiological diagnosis.J Endod. 2005; 31: 411-423
- Changes in root canal geometry after preparation assessed by high-resolution computed tomography.J Endod. 2001; 27: 1-6
- Bacterial community profiling of cryogenically ground samples from the apical and coronal root segments of teeth with apical periodontitis.J Endod. 2009; 35: 486-492
Article info
Publication history
Published online: November 17, 2020
Identification
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© 2020 American Association of Endodontists.
