Abstract
Introduction
The aim of this study was to compare the antibiofilm effects of a triple antibiotic
solution (TAS); a double antibiotic solution (DAS); and 5%, 2.5%, and 1.25% diclofenac
solutions (DCSs) against Enteroccocus faecalis biofilm.
Methods
Eighty-four sterile radicular dentin blocks were used as biofilm substrate for 3 weeks.
The study groups were as follows: (1) 1 mg/mL TAS (minocycline, metronidazole, and
ciprofloxacin), (2) 1 mg/mL DAS (metronidazole and ciprofloxacin), (3) 5% DCS, (4)
2.5% DCS, (5) 1.25% DCS, and (6) 0.9% saline solution. The antimicrobial activity
was evaluated by bacterial count determinations and confocal laser scanning microscopy.
The contact time for the antimicrobial tests was 5 minutes. Bacterial counts were
expressed as the reduction percentage of colony-forming units; for the confocal laser
scanning microscopic evaluation, the log10 total biovolume and percentage of green population (live cells) were calculated.
Results
The colony-forming unit reduction percentage ranged between 62.98 and 98.62, respectively,
for TAS and 5% DCS. The DCS showed a concentration-dependent effect.For the confocal
laser scanning microscopy, the log10 total biovolume in all groups was very similar and showed a scarce (1.39–1.02) but
significant reduction with respect to the control; 5% and 2.5% DCSs gave the lowest
viable cell percentage. The TAS and DAS groups showed intermediate values without
significant differences between them.
Conclusions
DCSs at 5% and 2.5% have greater antimicrobial effects than TAS and DAS and may be
considered a valid alternative for controlling the infection of teeth with apical
periodontitis.
Key Words
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Article info
Publication history
Published online: April 14, 2021
Identification
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© 2021 American Association of Endodontists.
