Abstract
Introduction
Guided tissue regeneration has been considered a promising biological strategy to
replace conventional endodontic therapies of teeth with incomplete root formation.
Therefore, in the present study, a collagen/gelatin hydrogel either containing dosages
of fibronectin (FN), or not, was developed and assessed concerning their bioactive
and chemotactic potential on human apical papilla cells (hAPCs).
Methods
Hydrogels were prepared by varying the ratio of collagen and gelatin (Col/Gel; v/v),
and used to establish the following groups: Collagen (positive control); Col/Gel 4:6;
Col/Gel 6:4; Col/Gel 8:2. The viability, adhesion, and spreading of cells seeded on
the hydrogels were evaluated. Different concentrations of FN (0, 5, or 10 μg/mL) were
incorporated into the best formulation of the collagen/gelatin hydrogel selected.
Then, the hAPCs seeded on the biomaterials were assessed concerning the cell migration,
viability, adhesion and spreading, and gene expression of ITGA5, ITGAV, COL1A1, and
COL3A1.
Results
The Col/Gel 8:2 group exhibited better cell viability, adhesion and spreading in comparison
with Control. Higher values of hAPC migration, viability, adhesion, spreading and
gene expression of pulp regeneration markers were found, the higher the concentration
was of FN incorporated into the collagen/gelatin hydrogel.
Conclusion
Collagen/gelatin hydrogel with 10 μg/mL of FN had potent bioactive and chemotactic
effects on cultured hAPCs.
Key Words
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Article info
Publication history
Published online: April 19, 2021
Identification
Copyright
© 2021 American Association of Endodontists.
