Abstract
Introduction
Human-derived composite amnion-chorion membrane (ACM) has been used for various regenerative
treatments. The aim of this pilot study was to investigate the effectiveness of the
ACM as a scaffold for pulp regeneration in mature canine teeth.
Methods
A total of 24 roots from mature premolars in dogs were included for regenerative procedures
using blood clots (BC) (group 1, n = 8), collagen membrane (CM) (group 2, n = 8), and ACM (group 3, n = 8). Each tooth was left open through a buccal access to induce root canal infection
and inflammation. The root canals were disinfected with 1.5% NaOCl and calcium hydroxide
intracanal medicament. After 2 weeks, bleeding was evoked to induce blood clot formation
(group 1) or before the placement of the membranes (groups 2 and 3). After 12 weeks,
the animals were euthanized for histologic assessment. The histologic data including
intracanal fibrous connective tissue, odontoblast-like cell lining, intracanal mineralized
tissue, periapical inflammation, and apical closure were qualitatively and quantitively
analyzed.
Results
Histologic analysis revealed that intracanal fibrous connective tissue was identified
in all groups, but a higher volume of the fibrous tissues was formed in the ACM group.
Odontoblast-like cells were only observed in the ACM group. The intracanal mineralized
tissue was observed only in the BC and CM groups. The BC group showed more periapical
inflammation than the ACM group (P < .05). Apical closure was more often found in the CM group than the BC group (P < .05).
Conclusions
More intracanal fibrous tissue and odontoblast-like cell lining, and less periapical
inflammation were observed after regenerative endodontic treatment in mature teeth
using the ACM than blood clot alone or blood clot with collagen membrane. The use
of the ACM may be useful for a cell-homing–based pulp regeneration in mature teeth.
Key Words
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Article info
Publication history
Published online: April 18, 2021
Identification
Copyright
© 2021 American Association of Endodontists.
