Abstract
Introduction
Semaphorin 7A (SEMA7A) is a membrane-bound or secretory protein exerting multiple
functions in the regulation of inflammation, neural degradation, and cancer progression.
Human periapical lesions are chronic and infectious diseases mainly caused by bacteria.
However, the involvement of SEMA7A in human periapical lesions is still unclear. This
study aimed to explore the expression of SEMA7A in human periapical lesions accompanied
by the potential association of SEMA7A with matrix metalloproteinase (MMP)-1 and MMP-3
during the progression of apical periodontitis.
Methods
Samples of periapical lesions and healthy controls were collected. Total RNA and protein
were extracted respectively for quantitative real-time polymerase chain reaction and
Western blot analysis. Additionally, 6 healthy samples and 27 periapical lesion samples
were fixed, dehydrated, and embedded for further histologic and immunochemical analysis.
The expression of SEMA7A was quantified by average integrated optical density. Immunofluorescence
analysis was conducted to explore the colocalization of SEMA7A/MMP-1 and SEMA7A/MMP-3.
Results
Compared with healthy controls, the messenger RNA and protein expression of SEMA7A
was markedly up-regulated in periapical lesions. A stronger expression of MMP-1, MMP-3,
and inflammatory cytokines was exhibited in periapical lesions than in healthy groups.
An increasing expression of SEMA7A can be observed in both the periapical granuloma
group and the radicular cyst group compared with the normal group (P < .01). Immunofluorescence results showed the colocalization of SEMA7A with both
MMP-1 and MMP-3 in vascular vessels and extracellular matrix.
Conclusions
SEMA7A was up-regulated in periapical periodontitis and might be involved in the tissue
destruction and infiltration of immune cells in periapical lesions.
Key Words
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Article info
Publication history
Published online: June 10, 2021
Identification
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© 2021 American Association of Endodontists.
