Abstract
Introduction
Mineral trioxide aggregate (MTA)-based sealers are endodontic materials with widespread
success in distinct clinical applications, potentially embracing direct contact with
the bone tissue. Bone response to these materials has been traditionally addressed
in vitro. Nonetheless, translational data are limited by the absence of native cell-to-cell
and cell-to-matrix interactions that hinder the representativeness of the analysis.
Ex vivo organotypic systems, relying on the culture of explanted biological tissues, preserve
the cell/tissue composition, reproducing the spatial and organizational in situ complexity. This study was grounded on an innovative research approach, relying on
the assessment of an ex vivo organotypic bone tissue culture system to address the osteogenic response to 3 distinct
MTA-based sealers.
Methods
Embryonic chick femurs were isolated and grown ex vivo for 11 days in the presence of MTA Plus (Avalon Biomed Inc, Bradenton, FL), ProRoot
MTA (Dentsply Tulsa Dental, Hohnson City, Germany), Biodentine (Septodont, Saint Maurdes
Fosses, France), or AH Plus (Dentsply Sirona, Konstanz, Germany); the latter was used
as a control material. Femurs were characterized by histologic, histochemical, and
histomorphometric analysis. Gene expression assessment of relevant osteogenic markers
was conducted by quantitative polymerase chain reaction.
Results
All MTA-based sealers presented an enhanced osteogenic performance compared with AH
Plus. Histochemical and histomorphometric analyses support the increased activation
of the osteogenic program by MTA-based sealers, with enhanced collagenous matrix deposition
and tissue mineralization. Gene expression analysis supported the enhanced activation
of the osteogenic program. Comparatively, ProRoot MTA induced the highest osteogenic
functionality on the characterized femurs.
Conclusions
MTA-based sealers enhanced the osteogenic activity within the assayed organotypic
bone model, which was found to be a sensitive system for the assessment of osteogenic
modulation mediated by endodontic sealers.
Key Words
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Article info
Publication history
Published online: June 10, 2021
Identification
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© 2021 American Association of Endodontists.
