Abstract
Introduction
Transient receptor potential ankyrin 1 (TRPA1) and vanilloid 1 (TRPV1) are thermosensitive
channels that play an important role in thermal sensation or tooth pain by regulating
intracellular Ca2+ concentration that is essential for pulp tissue repair. The aim of this study was
to evaluate the role of TRPA1 and TRPV1 channels in the odontogenic differentiation
of human dental pulp cells (HDPCs).
Methods
HDPCs were isolated from healthy human intact third molars and cultured in odontogenic
differentiation medium. Gene and protein expression levels of TRPA1 and TRPV1 channels
during the odontogenic differentiation of HDPCs were evaluated by real-time quantitative
polymerase chain reaction and Western blot analysis. HDPCs were then treated with
channel agonists or antagonists, and the expression levels of odontogenic markers
dentin sialophosphoprotein (DSPP) and osteopontin (OPN) were examined. Alkaline phosphatase
activity and alizarin red staining were also conducted to detect mineralization levels.
Results
Consistent with the mineralization degree and DSPP and OPN expression, messenger RNA
and protein expression of TRPA1 and TRPV1 channels was up-regulated during the odontogenic
differentiation of HDPCs. The application of TRPA1 or TRPV1 agonists increased the
mineralized nodules of alizarin red staining and alkaline phosphatase activity and
up-regulated the messenger RNA and protein expression of DSPP and OPN, respectively,
with the highest values reached on the seventh day (P < .05). On the contrary, the mineralization level and DSPP and OPN expression could
be suppressed by using the antagonists of these 2 channels.
Conclusions
TRPA1 and TRPV1 channels not only showed up-regulated expression along with the odontogenic
differentiation of HDPCs but also could affect the odontogenic differentiation by
regulating intracellular Ca2+ concentration.
Key Words
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Article info
Publication history
Published online: June 11, 2021
Identification
Copyright
© 2021 American Association of Endodontists.
