Abstract
The aim of this study was to develop a novel method of endodontic therapy, which we
refer to as dental pulp autotransplantation. Three patients (2 males and 1 female)
were selected for endodontic treatment of a uniradicular premolar and extraction of
a third molar (without odontosection). Electric assessment of pulp vitality and computed
tomographic imaging were undertaken followed by endodontic access and instrumentation
using triantibiotic solution for irrigation in the host tooth. A few minutes before
the transplant procedure, the third molar was extracted, the tooth was sectioned with
a diamond blade in a low-speed handpiece, and the pulp was carefully removed. After
premolar instrumentation, the harvested and preserved pulp tissue was reinserted into
the root canal followed by direct pulp capping performed using Biodentine (Septodont,
Saint-Maur-des-Fossés, France), a liner of resin-modified glass ionomer cement and
composite resin restoration. The teeth were followed up for at least 12 months after
the procedures and were analyzed using computed tomographic imaging, electric pulp
vitality testing, and Doppler ultrasound examination. At the 3- and 6-month follow-ups,
positive pulp vitality and regression of periapical lesions were verified. After 9–12 months,
all teeth were revascularized as determined by Doppler imaging, and the tooth vitality
was reestablished with no signs of endodontic/periodontal radiolucency or complications.
Within the limitations of the study, considering that it was a case series with only
3 patients, we described a highly innovative procedure of pulp autotransplantation,
which appears to be feasible, highlighting the potential for clinical application
of pulp regeneration using this new modality of endodontic therapy.
Key Words
To read this article in full you will need to make a payment
Purchase one-time access:
Academic & Personal: 24 hour online accessCorporate R&D Professionals: 24 hour online accessOne-time access price info
- For academic or personal research use, select 'Academic and Personal'
- For corporate R&D use, select 'Corporate R&D Professionals'
Subscribe:
Subscribe to Journal of EndodonticsAlready a print subscriber? Claim online access
Already an online subscriber? Sign in
Register: Create an account
Institutional Access: Sign in to ScienceDirect
References
- Animal models for stem cell-based pulp regeneration: foundation for human clinical applications.Tissue Eng Part B Rev. 2019; 25: 100-113
- Pulp regeneration in a full-length human tooth root using a hierarchical nanofibrous microsphere system.Acta Biomater. 2016; 35: 57-67
- A novel strategy to engineer pre-vascularized full-length dental pulp-like tissue constructs.Sci Rep. 2017; 7: 3323
- The application of bone morphogenetic proteins to dental tissue engineering.Nat Biotechnol. 2003; 21: 1025-1032
- GelMA-Encapsulated hDPSCs and HUVECs for dental pulp regeneration.J Dent Res. 2016; 96: 192-199
- Traumatized immature teeth treated with 2 protocols of pulp revascularization.J Endod. 2014; 40: 606-612
- Comparison of the effect of PRP, PRF and induced bleeding in the revascularization of teeth with necrotic pulp and open apex: a triple blind randomized clinical trial.J Clin Diagn Res. 2017; 11: ZC34-ZC39
- Assessment of regaining pulp sensibility in mature necrotic teeth using a modified revascularization technique with platelet-rich fibrin: a clinical study.J Endod. 2018; 44: 1526-1533
- Personalized cell therapy for pulpitis using autologous dental pulp stem cells and leukocyte platelet-rich fibrin: a case report.J Endod. 2019; 45: 144-149
- Autologous platelet concentrates for pulp and dentin regeneration: a literature review of animal studies.J Endod. 2016; 42: 250-257
- Pulp regeneration by transplantation of dental pulp stem cells in pulpitis: a pilot clinical study.Stem Cell Res Ther. 2017; 8: 61
- Minced pulp as source of pulpal mesenchymal stem cells with odontogenic differentiation capacity.J Endod. 2018; 44: 80-86
- Regenerative endodontic treatment as a retreatment option for a tooth with open apex - a case report.Braz Dent J. 2015; 26: 552-556
- Direct effect of intracanal medicaments on survival of stem cells of the apical papilla.J Endod. 2012; 38: 1372-1375
- Influence of age and apical diameter on the success of endodontic regeneration procedures.J Endod. 2016; 42: 1620-1625
- Dentin conditioning protocol for regenerative endodontic procedures.J Endod. 2020; 46: 1099-1104
- Effect of restorative timing on shear bond strength of composite resin/calcium silicate–based cements adhesive interfaces.Clin Oral Investig. 2020; 25: 3131-3139
- Autotransplantation of impacted third molar using 3D printing technology: a case report.Bull Tokyo Dent Coll. 2019; 60: 193-199
- Allogenic tooth transplantation using 3D printing: a case report and review of the literature.World J Clin Cases. 2019; 7: 2587-2596
- Pulp regeneration: current approaches, challenges, and novel rejuvenating strategies for an aging population.J Endod. 2020; 46: S135-S142
- Revascularization technique for the treatment of external inflammatory root resorption: a report of 3 cases.J Endod. 2015; 41: 1560-1564
- Comparative evaluation of the cytotoxic and angiogenic effects of minocycline and clindamycin: an in vitro study.J Endod. 2019; 45: 882-889
- Alternative to avoid tooth discoloration after regenerative endodontic procedure: a systematic review.Braz Dent J. 2018; 29: 409-418
- Doxycycline-loaded nanotube-modified adhesives inhibit MMP in a dose-dependent fashion.Clin Oral Investig. 2018; 22: 1243-1252
- Cytotoxicity and bioactivity of dental pulp-capping agents towards human tooth-pulp cells: a systematic review of in-vitro studies and meta-analysis of randomized and controlled clinical trials.Materials (Basel). 2020; 13: 2670
- Degradation of adhesive-dentin interfaces created using different bonding strategies after five-year simulated pulpal pressure.J Adhes Dent. 2019; 21: 199-207
- From Buonocore's pioneering acid-etch technique to self-adhering restoratives. A status perspective of rapidly advancing dental adhesive technology.J Adhes Dent. 2020; 22: 7-34
Article info
Publication history
Published online: June 23, 2021
Identification
Copyright
© 2021 American Association of Endodontists.
