Abstract
Introduction
Molecular diagnosis may overcome the limitations of clinical and histologic diagnosis
in pulpitis, thereby benefiting many treatment techniques, such as vital pulp therapies.
In this study, integrated microarray data on pulpitis were used to obtain a list of
normalized differentially expressed (DE) genes for analyzing the molecular mechanisms
underlying pulpitis and identifying potential diagnostic biomarkers.
Methods
A systematic search of public microarray and sequencing databases was performed to
obtain expression data of pulpitis. Robust rank aggregation (RRA) was used to obtain
DE gene lists (RRA_DEmRNAs and RRA_DElncRNAs) between inflamed pulp and normal samples.
DE genes were evaluated by functional enrichment analyses, correlation analyses for
inflammation-related RRA_DEmRNAs, and protein-protein interaction and competing endogenous
RNA network construction. Quantitative real-time polymerase chain reaction validation
was applied in snap-frozen pulp tissues.
Results
Using the GSE77459 and GSE92681 data sets, 280 RRA_DEmRNAs and 90 RRA_DElncRNAs were
identified. RRA_DEmRNAs were significantly enriched in inflammation-related biological
processes and osteoclast differentiation and tumor necrosis factor, chemokine, and
B-cell receptor signaling pathways. The molecular complex detection and cytoHubba
methods identified 2 clusters and 10 hub genes in the protein-protein interaction
network. The competing endogenous RNA network was composed of 2 long noncoding RNAs
(ADAMTS9-AS2 and LINC00290), 2 microRNAs (hsa-miR-30a-5p and hsa-miR-128-3p), and 3 messenger RNAs (ABCA1, FBLN5, and SOCS3). The expression between most top inflammation-related RRA_DEmRNAs in pulpitis showed
positive correlations. Quantitative real-time polymerase chain reacation validated
the expression trends of selected genes, including ITGAX, TREM1, CD86, FCGR2A, ADAMTS9-AS2, LINC00290, hsa-miR-30a-5p, hsa-miR-128-3p, RASGRP3, IL3RA, CCDC178, CRISPLD1, LINC01857, AC007991.2, ARHGEF26-AS1, and AL021408.1.
Conclusions
The identified biomarkers provide insight into the pathology and may aid in the molecular
diagnosis of pulpitis.
Key Words
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Article info
Publication history
Published online: July 11, 2021
Identification
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© 2021 American Association of Endodontists.
