Abstract
Introduction
The purpose of this study was to assess the antimicrobial activity of root canal sealers
modified with novel highly loaded antimicrobial drug-silica coassembled particles
(DSPs) on Enterococcus faecalis–infected root canal dentin.
Methods
DSPs were synthesized through coassembly of silica and octenidine dihydrochloride
(OCT) surfactant drug (35% w/w OCT). DSPs (1% wt of the total mass of the sealer)
were mixed homogenously with either epoxy resin sealer (AH Plus [AH]; Dentsply Sirona,
Tulsa, OK) or calcium silicate–based sealer (EndoSequence BC Sealer [BC]; Brasseler,
Savannah, GA). To assess the antimicrobial activity of DSP-loaded sealers, the apical
third of single-rooted teeth was obtained and infected with E. faecalis for 3 weeks followed by the application of experimental (DSP-loaded) sealers or corresponding
controls for up to 28 days. Microbiological analysis and laser scanning confocal and
scanning electron microscopy were used to determine the colony-forming unit (CFU)/mL,
the percentage of live bacteria, and the intratubular bacterial and sealer penetrations.
Factorial analysis of variance and Tukey post hoc tests were used to assess the antimicrobial
effect of DSPs on different sealers.
Results
All experimental groups showed significant reductions in CFUs at all-time points compared
with positive controls (P < .05). The addition of DSPs to BC significantly reduced the CFUs (2.11 ± 0.13, 2.22 ± 0.19,
and 2.25 ± 0.17 at 1, 7, and 28 days, respectively) compared with the unmodified sealer
(3.21 ± 0.11, 4.3 ± 0.15, and 4.2 ± 0.2 at 0, 7, and 28 days). DSPs enhanced the antimicrobial
performance of AH only at 1 day (4.21 ± 0.17 vs 5.19 ± 0.12, P < .05). AH and AH + DSPs showed higher bacterial viability compared with BC and BC + DSPs
at all incubation periods (P < .05).
Conclusions
Loading endodontic sealers with DSPs had a material-dependent effect on the antimicrobial
properties and could reduce the incidence of secondary infections.
Key Words
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Article info
Publication history
Published online: August 20, 2021
Identification
Copyright
© 2021 American Association of Endodontists.
