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Basic Research| Volume 47, ISSUE 11, P1775-1782, November 2021

Assessment of Root Canal Sealers Loaded with Drug-Silica Coassembled Particles Using an In Vitro Tooth Model

Published:August 20, 2021DOI:https://doi.org/10.1016/j.joen.2021.08.005

      Abstract

      Introduction

      The purpose of this study was to assess the antimicrobial activity of root canal sealers modified with novel highly loaded antimicrobial drug-silica coassembled particles (DSPs) on Enterococcus faecalis–infected root canal dentin.

      Methods

      DSPs were synthesized through coassembly of silica and octenidine dihydrochloride (OCT) surfactant drug (35% w/w OCT). DSPs (1% wt of the total mass of the sealer) were mixed homogenously with either epoxy resin sealer (AH Plus [AH]; Dentsply Sirona, Tulsa, OK) or calcium silicate–based sealer (EndoSequence BC Sealer [BC]; Brasseler, Savannah, GA). To assess the antimicrobial activity of DSP-loaded sealers, the apical third of single-rooted teeth was obtained and infected with E. faecalis for 3 weeks followed by the application of experimental (DSP-loaded) sealers or corresponding controls for up to 28 days. Microbiological analysis and laser scanning confocal and scanning electron microscopy were used to determine the colony-forming unit (CFU)/mL, the percentage of live bacteria, and the intratubular bacterial and sealer penetrations. Factorial analysis of variance and Tukey post hoc tests were used to assess the antimicrobial effect of DSPs on different sealers.

      Results

      All experimental groups showed significant reductions in CFUs at all-time points compared with positive controls (P < .05). The addition of DSPs to BC significantly reduced the CFUs (2.11 ± 0.13, 2.22 ± 0.19, and 2.25 ± 0.17 at 1, 7, and 28 days, respectively) compared with the unmodified sealer (3.21 ± 0.11, 4.3 ± 0.15, and 4.2 ± 0.2 at 0, 7, and 28 days). DSPs enhanced the antimicrobial performance of AH only at 1 day (4.21 ± 0.17 vs 5.19 ± 0.12, P < .05). AH and AH + DSPs showed higher bacterial viability compared with BC and BC + DSPs at all incubation periods (P < .05).

      Conclusions

      Loading endodontic sealers with DSPs had a material-dependent effect on the antimicrobial properties and could reduce the incidence of secondary infections.

      Key Words

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      References

        • Xu C.
        • Lei C.
        • Yu C.
        Mesoporous silica nanoparticles for protein protection and delivery.
        Front Chem. 2019; 7: 290
        • Stewart C.A.
        • Finer Y.
        • Hatton B.D.
        Drug self-assembly for synthesis of highly-loaded antimicrobial drug-silica particles.
        Sci Rep. 2018; 8: 895
        • Rohrer N.
        • Widmer A.F.
        • Waltimo T.
        • et al.
        Antimicrobial efficacy of 3 oral antiseptics containing octenidine, polyhexamethylene biguanide, or Citroxx: can chlorhexidine be replaced?.
        Infect Control Hosp Epidemiol. 2010; 31: 733-739
        • Varghese V.S.
        • Uppin V.
        • Bhat K.
        • et al.
        Antimicrobial efficacy of octenidine hydrochloride and calcium hydroxide with and without a carrier: a broth dilution analysis.
        Contemp Clin Dent. 2018; 9: 72-76
        • de Lucena J.M.
        • Decker E.M.
        • Walter C.
        • et al.
        Antimicrobial effectiveness of intracanal medicaments on Enterococcus faecalis: chlorhexidine versus octenidine.
        Int Endod J. 2013; 46: 53-61
        • Stuart C.H.
        • Schwartz S.A.
        • Beeson T.J.
        • Owatz C.B.
        Enterococcus faecalis: its role in root canal treatment failure and current concepts in retreatment.
        J Endod. 2006; 32: 93-98
        • Zoletti G.O.
        • Siqueira J.F.
        • Santos K.R.
        Identification of Enterococcus faecalis in root-filled teeth with or without periradicular lesions by culture-dependent and—independent approaches.
        J Endod. 2006; 32: 722-726
        • Evans M.
        • Davies J.K.
        • Sundqvist G.
        • Figdor D.
        Mechanisms involved in the resistance of Enterococcus faecalis to calcium hydroxide.
        Int Endod J. 2002; 35: 221-228
        • Nair U.
        • Ghattas S.
        • Saber M.
        • et al.
        A comparative evaluation of the sealing ability of 2 root-end filling materials: an in vitro leakage study using Enterococcus faecalis.
        Oral Surg Oral Med Oral Pathol Oral Radiol Endod. 2011; 112: e74-e77
        • Peters O.A.
        • Schonenberger K.
        • Laib A.
        Effects of four Ni-Ti preparation techniques on root canal geometry assessed by micro computed tomography.
        Int Endod J. 2001; 34: 221-230
        • Sjogren U.
        • Figdor D.
        • Persson S.
        • Sundqvist G.
        Influence of infection at the time of root filling on the outcome of endodontic treatment of teeth with apical periodontitis.
        Int Endod J. 1997; 30: 297-306
        • Spångberg L.S.
        • Haapasalo M.
        Rationale and efficacy of root canal medicaments and root filling materials with emphasis on treatment outcome.
        Endod Topics. 2002; 2: 35-58
        • Del Carpio-Perochena A.
        • Kishen A.
        • Shrestha A.
        • Bramante C.M.
        Antibacterial properties associated with chitosan nanoparticle treatment on root dentin and 2 types of endodontic sealers.
        J Endod. 2015; 41: 1353-1358
        • Zhang H.
        • Shen Y.
        • Ruse N.D.
        • Haapasalo M.
        Antibacterial activity of endodontic sealers by modified direct contact test against Enterococcus faecalis.
        J Endod. 2009; 35: 1051-1055
        • Shrestha A.
        • Kishen A.
        Antibacterial nanoparticles in endodontics: a review.
        J Endod. 2016; 42: 1417-1426
        • Kishen A.
        Nanotechnology in Endodontics.
        Springer, New York2016
        • Kim Y.K.
        • Grandini S.
        • Ames J.M.
        • et al.
        Critical review on methacrylate resin-based root canal sealers.
        J Endod. 2010; 36: 383-399
        • Huang T.-H.
        • Yang J.-J.
        • Li H.
        • Kao C.-T.
        The biocompatibility evaluation of epoxy resin-based root canal sealers in vitro.
        Biomaterials. 2002; 23: 77-83
        • Gomes B.P.
        • Pedroso J.A.
        • Jacinto R.C.
        • et al.
        In vitro evaluation of the antimicrobial activity of five root canal sealers.
        Braz Dent J. 2004; 15: 30-35
        • Silva Almeida L.H.
        • Moraes R.R.
        • Morgental R.D.
        • Pappen F.G.
        Are premixed calcium silicate-based endodontic sealers comparable to conventional materials? A systematic review of in vitro studies.
        J Endod. 2017; 43: 527-535
        • Urban K.
        • Neuhaus J.
        • Donnermeyer D.
        • et al.
        Solubility and pH value of 3 different root canal sealers: a long-term investigation.
        J Endod. 2018; 44: 1736-1740
        • Marashdeh M.Q.
        • Friedman S.
        • Lévesque C.
        • Finer Y.
        Esterases affect the physical properties of materials used to seal the endodontic space.
        Dent Mater. 2019; 35: 1065-1072
        • Chybowski E.A.
        • Glickman G.N.
        • Patel Y.
        • et al.
        Clinical outcome of non-surgical root canal treatment using a single-cone technique with Endosequence bioceramic sealer: a retrospective analysis.
        J Endod. 2018; 44: 941-945
        • Marashdeh M.
        • Stewart C.
        • Kishen A.
        • et al.
        Drug-silica co-assembled particles improve antimicrobial properties of endodontic sealers.
        J Endod. 2021; 47: 793-799
        • Haapasalo H.K.
        • Siren E.K.
        • Waltimo T.M.
        • et al.
        Inactivation of local root canal medicaments by dentine: an in vitro study.
        Int Endod J. 2000; 33: 126-131
        • Titley K.C.
        • Chernecky R.
        • Rossouw P.E.
        • Kulkarni G.V.
        The effect of various storage methods and media on shear-bond strengths of dental composite resin to bovine dentine.
        Arch Oral Biol. 1998; 43: 305-311
        • Shi Y.
        • Miller M.L.
        • Di Pasqua A.J.
        Biocompatibility of mesoporous silica nanoparticles?.
        Comments Inorg Chem. 2016; 36: 61-80
        • Nair P.N.
        On the causes of persistent apical periodontitis: a review.
        Int Endod J. 2006; 39: 249-281
        • Stojicic S.
        • Shen Y.
        • Haapasalo M.
        Effect of the source of biofilm bacteria, level of biofilm maturation, and type of disinfecting agent on the susceptibility of biofilm bacteria to antibacterial agents.
        J Endod. 2013; 39: 473-477
        • Wang Z.
        • Shen Y.
        • Haapasalo M.
        Effectiveness of endodontic disinfecting solutions against young and old Enterococcus faecalis biofilms in dentin canals.
        J Endod. 2012; 38: 1376-1379
        • Sedgley C.M.
        • Lennan S.L.
        • Appelbe O.K.
        Survival of Enterococcus faecalis in root canals ex vivo.
        Int Endod J. 2005; 38: 735-742
        • Kishen A.
        • Shrestha A.
        • Del Carpio-Perochena A.
        Validation of biofilm assays to assess antibiofilm efficacy in instrumented root canals after syringe irrigation and sonic agitation.
        J Endod. 2018; 44: 292-298
        • Kishen A.
        • Haapasalo M.
        Biofilm models and methods of biofilm assessment.
        Endod Topics. 2010; 22: 58-78
        • Little B.J.
        • Ray R.I.
        • Pope R.
        • Scheetz R.
        Biofilms: an ESEM evaluation of artifacts introduced during SEM preparation.
        J Ind Microbiol. 1992; 8: 213-222
        • Gong S.-Q.
        • Huang Z.-b.
        • Shi W.
        • et al.
        In vitro evaluation of antibacterial effect of AH Plus incorporated with quaternary ammonium epoxy silicate against Enterococcus faecalis.
        J Endod. 2014; 40: 1611-1615
        • Barros J.
        • Silva M.G.
        • Rodrigues M.A.
        • et al.
        Antibacterial, physicochemical and mechanical properties of endodontic sealers containing quaternary ammonium polyethylenimine nanoparticles.
        Int Endod J. 2014; 47: 725-734
        • Uzunoglu-Özyürek E.
        • Erdoğan Ö.
        • Aktemur Türker S.
        Effect of calcium hydroxide dressing on the dentinal tubule penetration of 2 different root canal sealers: a confocal laser scanning microscopic study.
        J Endod. 2018; 44: 1018-1023