Abstract
Introduction
Appropriate occlusal forces can prevent ankylosis after tooth replantation or transplantation.
However, the “proper occlusal forces” on periodontal ligament (PDL) healing have not
yet been defined due to insufficient in vitro studies and uncertain in vitro models. Herein, we presented a mechanical vibration device as an in vitro model to determine such favorable occlusal forces.
Methods
Human periodontal ligament stem cells (hPDLSCs) were exposed to mechanical vibration
force with 4 frequencies (30, 90, 150, and 210 rpm). Cell viability and the expression
of osteogenic differentiation-related genes and proteins were tested in vitro. The calvarial transplantation experiment was performed to assess the bone formation
ability of 150 rpm mechanical vibration stimulation (MVS).
Results
MVS at 150 and 210 rpm significantly reduced cell viability in the early stages. The
150-rpm MVS decreased osteogenic marker expression at the early time point (3 days)
but had no harmful effects at the late time point (14 days). Furthermore, hPDLSC cell
sheets treated with 150-rpm MVS had potential to decrease bone formation in rat calvarial
defects serendipitously and facilitated functional PDL-like tissue formation.
Conclusions
We found that MVS at a frequency of 150 rpm could provide a strategy for a transient
reduction in the osteogenic potential of hPDLSCs and promote PDL-like tissue formation.
Thus, 150-rpm MVS could be used as a controllable proper occlusal force to prevent
ankylosis and promote PDL healing after tooth replantation or transplantation.
Key Words
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Article info
Publication history
Published online: September 04, 2021
Identification
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© 2021 American Association of Endodontists.
