Appropriate occlusal forces can prevent ankylosis after tooth replantation or transplantation. However, the “proper occlusal forces” on periodontal ligament (PDL) healing have not yet been defined due to insufficient in vitro studies and uncertain in vitro models. Herein, we presented a mechanical vibration device as an in vitro model to determine such favorable occlusal forces.
Human periodontal ligament stem cells (hPDLSCs) were exposed to mechanical vibration force with 4 frequencies (30, 90, 150, and 210 rpm). Cell viability and the expression of osteogenic differentiation-related genes and proteins were tested in vitro. The calvarial transplantation experiment was performed to assess the bone formation ability of 150 rpm mechanical vibration stimulation (MVS).
MVS at 150 and 210 rpm significantly reduced cell viability in the early stages. The 150-rpm MVS decreased osteogenic marker expression at the early time point (3 days) but had no harmful effects at the late time point (14 days). Furthermore, hPDLSC cell sheets treated with 150-rpm MVS had potential to decrease bone formation in rat calvarial defects serendipitously and facilitated functional PDL-like tissue formation.
We found that MVS at a frequency of 150 rpm could provide a strategy for a transient reduction in the osteogenic potential of hPDLSCs and promote PDL-like tissue formation. Thus, 150-rpm MVS could be used as a controllable proper occlusal force to prevent ankylosis and promote PDL healing after tooth replantation or transplantation.
To read this article in full you will need to make a payment
Purchase one-time access:Academic & Personal: 24 hour online accessCorporate R&D Professionals: 24 hour online access
One-time access price info
- For academic or personal research use, select 'Academic and Personal'
- For corporate R&D use, select 'Corporate R&D Professionals'
Subscribe:Subscribe to Journal of Endodontics
Already a print subscriber? Claim online access
Already an online subscriber? Sign in
Register: Create an account
Institutional Access: Sign in to ScienceDirect
- Periodontal healing after replantation and autotransplantation of incisors in monkeys.Int J Oral Surg. 1981; 10: 54-61
- The tissue, cellular, and molecular regulation of orthodontic tooth movement: 100 years after Carl Sandstedt.Eur J Orthod. 2006; 28: 221-240
- Alveolar bone turnover and periodontal ligament width are controlled by Wnt.J Periodontol. 2015; 86: 319-326
- Effect of early orthodontic force on periodontal healing after autotransplantation of permanent incisors in beagle dogs.J Periodontol. 2012; 83: 235-241
- Effect of masticatory stimulation on dentoalveolar ankylosis after experimental tooth replantation.Dent Traumatol. 1985; 1: 13-16
- Effects of occlusal loading on ankylosis, bone, and cementum formation during bone morphogenetic protein-2-stimulated periodontal regeneration in vivo.J Periodontol. 1999; 70: 1125-1135
- Occlusal forces promote periodontal healing of transplanted teeth and prevent dentoalveolar ankylosis: an experimental study in rats.Angle Orthod. 2005; 75: 637-644
- The effect of excessive occlusal trauma upon periodontal healing after replantation of mature permanent incisors in monkeys.Swed Dent J. 1981; 5: 115-122
- Effect of mechanical loading on periodontal cells.Crit Rev Oral Biol Med. 2001; 12: 414-424
- Stem cells and periodontal regeneration.Periodontol 2000. 2006; 40: 164-172
- Mechanoadaptive responses in the periodontium are coordinated by Wnt.J Dent Res. 2019; 98: 689-697
- Molecular basis for periodontal ligament adaptation to in vivo loading.J Dent Res. 2019; 98: 331-338
- Osteogenic gene expression by human periodontal ligament cells under cyclic tension.J Dent Res. 2007; 86: 1212-1216
- Effects of mechanical vibration on proliferation and osteogenic differentiation of human periodontal ligament stem cells.Arch Oral Biol. 2012; 57: 1395-1407
- In vitro mechanical loading models for periodontal ligament cells: from two-dimensional to three-dimensional models.Arch Oral Biol. 2015; 60: 416-424
- Vibratory stimulus and accelerated tooth movement: a critical appraisal.J World Fed Orthod. 2018; 7: 106-112
- Retention and healing outcomes after intentional replantation.J Endod. 2016; 42: 909-915
- Three-dimensional loading model for periodontal ligament regeneration in vitro.Tissue Eng Part C Methods. 2009; 15: 561-570
- Influence of different intensities of vibration on proliferation and differentiation of human periodontal ligament stem cells.Arch Med Sci. 2015; 11: 638-646
- Effects of low magnitude high frequency mechanical vibration combined with compressive force on human periodontal ligament cells in vitro.Eur J Orthod. 2018; 40: 356-363
- Recent advances in periodontal regeneration: a biomaterial perspective.Bioact Mater. 2020; 5: 297-308
- Periodontal ligament cell sheet promotes periodontal regeneration in athymic rats.J Clin Periodontol. 2008; 35: 1066-1072
- Periodontal healing by periodontal ligament cell sheets in a teeth replantation model.Arch Oral Biol. 2012; 57: 169-176
- Cell sheet engineering and its application for periodontal regeneration.J Tissue Eng Regen Med. 2015; 9: 343-356
Published online: September 04, 2021
© 2021 American Association of Endodontists.