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EVALUATION OF RANKL, OPG, OPN AND TNF-α ON CHRONIC APICAL PERIODONTITIS IN SMOKERS

  • K.M. De Paula
    Affiliations
    Postgraduate Program in Dentistry, Dental Clinic concentration area, FFE Department, Nova Friburgo Health Institute, Fluminense Federal University, Nova Friburgo, RJ, Brazil
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  • C.C. Gomes
    Correspondence
    Correspondence to Dr. Cinthya Cristina Gomes, Department of Specific Formation, College of Dentistry, Fluminense Federal University, Nova Friburgo, RJ, Brazil. Rua Doutor Silvio Henrique Braune, 22 – Centro, Nova Friburgo, Rio de Janeiro Brazil - CEP: 28625-650. Fax: +55-22-25287168.
    Affiliations
    Postgraduate Program in Dentistry, Dental Clinic concentration area, FFE Department, Nova Friburgo Health Institute, Fluminense Federal University, Nova Friburgo, RJ, Brazil
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  • M.I.B. Valente
    Affiliations
    Postgraduate Program in Dentistry, Dental Clinic concentration area, FFE Department, Nova Friburgo Health Institute, Fluminense Federal University, Nova Friburgo, RJ, Brazil
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  • F.R. Pires
    Affiliations
    Postgraduate Program in Dentistry, Endodontics, Estácio de Sá University, Rio de Janeiro, RJ, Brazil
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  • K.A.B.R. Thuller
    Affiliations
    Postgraduate Program in Dentistry, Dental Clinic concentration area, FFE Department, Nova Friburgo Health Institute, Fluminense Federal University, Nova Friburgo, RJ, Brazil
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  • L. Salles
    Affiliations
    Postgraduate Program in Dentistry, Dental Clinic concentration area, FFE Department, Nova Friburgo Health Institute, Fluminense Federal University, Nova Friburgo, RJ, Brazil
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  • L. Armada
    Affiliations
    Postgraduate Program in Dentistry, Endodontics, Estácio de Sá University, Rio de Janeiro, RJ, Brazil
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Published:November 18, 2022DOI:https://doi.org/10.1016/j.joen.2022.11.012

      ABSTRACT

      Introduction

      Smoking can be considered a risk factor for Chronic Apical Periodontitis (CAP). This study compared the immunoexpression of biomarkers RANKL, OPG, OPN and TNF- α in CAP in smokers and non-smokers.

      Methods

      Twelve smokers (S) and twelve non-smokers (NS) diagnosed with CAP and indicated for extraction were selected. Exclusion factors were teeth with a diagnosis of root fracture, previous endodontic treatment and endoperiodontal injury, in addition to individuals with systemic diseases, under 18 years of age, users of anti-inflammatory and/or antibiotics in the last three months and drug users. Specimens were processed for histopathologic and immunohistochemical analysis.

      Results

      The qualitative analysis of RANKL expression showed 66.66% weak/moderate and 33.33% strong in S and 100% weak/moderate in the NS. OPG and OPN expressions were 100% negative to focal in the S group and 50% negative to focal, 50% weak/moderate in the NS. TNF- α was 25% negative to focal and 75% weak/moderate in the S and 33.33% negative to focal and 66.66% weak/moderate in the NS. The quantitative analysis of the data by the Mann-Whitney test, showed that was a significant difference in the immunoexpression of RANKL (p<0.05), OPG (p<0.05) and OPN (p<0.05) but there was no statistical difference in the immunoexpression of TNF- α (p>0.05) between the two groups.

      Conclusions

      These findings suggest that smoking is capable of altering the inflammatory response, influencing the evolution of CAP.

      Keywords

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