Abstract
Introduction
Intracanal medicament is one of the essential steps for ensuring success in regenerative
endodontic procedures. L-Chg10-teixobactin is a novel antimicrobial agent that exhibited potent antibacterial and
antibiofilm effects against Enterococcus faecalis at low concentrations compared with ampicillin. At the same time, its cytotoxicity
on dental stem cells has not been studied. This study aimed to investigate the effects
of L-Chg10-teixobactin on the viability, proliferation, migration, and osteo/odontogenic differentiation
of stem cells from apical papilla (SCAPs).
Materials and Methods
SCAPs isolated from immature human third molars were treated with various concentrations
of L-Chg10-teixobactin, calcium hydroxide, and dimethyl sulfoxide. The viability and proliferation
of SCAPs were assessed using the LIVE/DEAD Viability/Cytotoxicity Kit and Cell Counting
Kit-8. A scratch wound healing test was used to evaluate the lateral migration capacity
of SCAPs. Alkaline phosphatase (ALP) activity, calcium mineralization ability tests
-ie, ALP staining and alizarin red S staining, and quantitative real-time polymerase
chain reaction were performed to assess the osteo /odontogenic differentiation of
SCAPs.
Results
The tested concentrations of L-Chg10-teixobactin (0.01, 0.02, and 0.03 mg/mL), 1 mg/mL calcium hydroxide, and 0.03% dimethyl
sulfoxide had no significant cytotoxic effect on SCAPs at any time point (P > .05). Besides, there were no significant differences between the control and experimental
groups in SCAPs’ viability, proliferation, and migration. L-Chg10-teixobactin upregulated the gene expression of osteo/odontogenic markers in SCAPs,
while no significant difference was found in the ALP activity and alizarin red S staining.
Conclusions
L-Chg10-teixobactin demonstrated excellent biocompatibility on SCAPs at concentrations from
0.01 to 0.03 mg/mL and potentially enhance the osteo/odontogenic differentiation of
SCAPs; suggesting its promising role as root canal medicament for regenerative endodontic
procedures.
Key Words
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Article info
Publication history
Published online: December 30, 2022
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© 2022 American Association of Endodontists.
