Abstract
Introduction
Apical periodontitis (AP) represents an inflammatory condition of peri-radicular tissues
due to invasion and colonization of bacteria in the root canals. Primary apical periodontitis
(PAP) is associated with untreated necrotic root canal and can be efficiently treated
with endodontic treatment to remove bacteria. Persistent/secondary apical periodontitis
(SAP) is a perpetual periapical lesion due to unsuccessfully treated root canals after
an initial apparent healing of the tooth. The aim of the study was evaluating the
microbial communities associated with root canals using Nanopore sequencing.
Methods
Seventeen samples from the root canals of 15 patients with AP were Polymerase Chain
Reaction-amplified for 16s ribosomal DNA gene and sequenced. Information regarding
the presence or absence of AP symptoms, PAP and SAP, and periapical index of patients
were recorded.
Results
Firmicutes, Bacteroidetes, and Actinobacteria were the most abundant phyla detected
and Phocaeicola, Pseudomonas, Rothia, and Prevotella were the most prominent genera. In samples of patients with AP symptoms, the most
frequent detected genera were Cutibacterium, Lactobacillus, Pseudomonas, Dialister, Prevotella, and Staphylococcus. In PAP samples, the most represented genera were Cutibacterium, Lactobacillus, Pseudomonas, and Prevotella, whilst in SAP cases were Cutibacterium, Prevotella, Atopobium, Capnocytophaga, Fusobacterium, Pseudomonas, Solobacterium, and Streptococcus.
Conclusions
The results provide additional information on the microbiota of root-canals. These
data evidence the complexity of the microbiota and the relationship with many clinical
and endodontic conditions. Future studies must evaluate these conditions and identify
their role in inducing bone damage and local and systemic disease, aiming to better
elucidate the relationship between microbes and endodontic pathologies.
Key Words
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Article info
Publication history
Published online: December 13, 2022
Footnotes
Ethical approval: The Ethics Committee of Azienda Unità Sanitaria Locale of Bologna approved this study with authorization nr. 844-2021-OSS-AUSLBO-21160-ID 3118-Parere CE-AVEC-ENDO-MICROBIOTA 09/2021.
Identification
Copyright
© 2022 American Association of Endodontists.
