The aim of this study was to assess whether the biological characteristics of dental pulp stem cells (DPSCs), such as viability, adhesion to dentin, mineralization, and release of immunomodulatory cytokines, are affected by the inflammatory status of the donor tissue and/or the sustained inflammatory environment.
DPSCs were isolated from pulps from 3 caries-free teeth (healthy or hDPSCs), and from 3 teeth with irreversible pulpitis or deep caries (unhealthy DPSCs or uDPSCs). The cells were cultured in odontogenic and osteogenic media with or without lipopolysaccharides. Viability was analyzed by MTT assay at days 1, 3, 5, and 7; adhesion to dentin was evaluated through an environmental scanning electron microscope after 48 hours and through MTT assay; mineralization was analyzed with alizarin red staining after 21 days; and the release of proinflammatory (interleukin 6) and immunosuppressive cytokines (interleukin 10) was measured with the enzyme-linked immunosorbent assay after 24 hours and 7 days.
The inflammatory status of the pulp significantly reduced the viability and mineralization capacity of the DPSCs, although it did not affect the adhesion capacity to dentin or the secretion of the proinflammatory interleukin. The inflammatory microenvironment (lipopolysaccharide) only had a significant impact on the secretion of interleukin 6, which was augmented after 7 days.
The inflammatory status of the dental pulp should be taken into account when the use of DPSCs is intended either for research and/or for application in reparative or regenerative therapies.
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- An overview of the dental pulp: its functions and responses to injury.Aust Dent J. 2007; 52: S4-S6
- Postnatal human dental pulp stem cells (DPSCs) in vitro and in vivo.Proc Natl Acad Sci U S A. 2000; 97: 13625-13630
- Dentin: structure, composition and mineralization.Front Biosci. 2011; 3: 711-735
- Effect of isolation methodology on stem cell properties and multilineage differentiation potential of human dental pulp stem cells.Cell Tissue Res. 2013; 353: 65-78
- Microbial modulation of stem cells and future directions in regenerative endodontics.J Endod. 2017; 43: S95-S101
- Effect of bacterial biofilm on the osteogenic differentiation of stem cells of apical papilla.J Endod. 2017; 43: 916-922
- Pulp and dentine responses to selective caries excavation: a histological and histobacteriological human study.J Dent. 2020; 100: 103430
- Reactionary dentinogenesis and neuroimmune response in dental caries.J Dent Res. 2014; 93: 788-793
- Mediators of inflammation and regeneration.Adv Dent Res. 2011; 23: 290-295
- Pulp inflammation diagnosis from clinical to inflammatory mediators: a systematic review.J Endod. 2017; 43: 1033-1051
- Up-regulation of IL-23 expression in human dental pulp fibroblasts by IL-17 via activation of the NF-kappaB and MAPK pathways.Int Endod J. 2018; 51: 622-631
- Inflammation and regeneration in the dentin-pulp complex: A double- edged sword.J Endod. 2014; 40: S46-S51
- Pattern Recognition Receptors and Inflammation.Cell. 2010; 140: 805-820
- Interactive effects of LPS and dentine matrix proteins on human dental pulp stem cells.Int Endod J. 2018; 51: 877-888
- Mesenchymal stem cells derived from human inflamed dental pulp exhibit impaired immunomodulatory capacity in vitro.J Endod. 2020; 46: 1091-1098
- Potentiality and inflammatory marker expression are maintained in dental pulp cell cultures from carious teeth.Int J Mol Sci. 2022; 23: 9425
- Is hard tissue formation in the dental pulp after the death of the primary odontoblasts a regenerative or a reparative process?.J Dent. 2014; 42: 1156-1170
- Changes in proliferation and osteogenic differentiation of stem cells from deep caries in vitro.J Endod. 2012; 38: 796-802
- Comparison of stem cell properties of cells isolated from normal and inflamed dental pulps.Int Endod J. 2012; 45: 1080-1090
- The effects of LPS on adhesion and migration of human dental pulp stem cells in vitro.J Dent. 2014; 42: 1327-1334
- Differential effects of Escherichia coli versus Porphyromonas gingivalis-derived lipopolysaccharides on dental pulp stem cell differentiation in scaffold-free engineered tissues.J Endod. 2022; 48: 1378-1386
- EDTA conditioning of dentine promotes adhesion, migration and differentiation of dental pulp stem cells.Int Endod J. 2016; 49: 581-590
- Shotgun proteomics of human dentin with different prefractionation methods.Sci Rep. 2019; 9: 4457
- Pulp and apical tissue response to deep caries in immature teeth: A histologic and histobacteriologic study.J Dent. 2017; 56: 19-32
- Changes in the radicular pulp-dentine complex in healthy intact teeth and in response to deep caries or restorations: A histological and histobacteriological study.J Dent. 2018; 73: 76-90
- IL-6 as a keystone cytokine in health and disease.Nat Immunol. 2015; 16: 448-457
- Dental pulp defence and repair mechanisms in dental caries.Mediators Inflamm. 2015; 2015: 230251
- Infection of human dental pulp stromal cells by Streptococcus mutans: shedding light on bacteria pathogenicity and pulp inflammation.Front Cell Dev Biol. 2020; 31: 785
- Different expression patterns of inflammatory cytokines induced by lipopolysaccharides from Escherichia coli or Porphyromonas gingivalis in human dental pulp stem cells.BMC Oral Health. 2022; 22: 121
- Cytokine production by human odontoblast-like cells upon Toll-like receptor-2 engagement.Immunobiology. 2011; 216: 513-517
- Pattern-recognition receptors in pulp defense.Adv Dent Res. 2011; 23: 296-301
- TLR4 activation by lipopolysaccharide and Streptococcus mutans induces differential regulation of proliferation and migration in human dental pulp stem cells.J Endod. 2014; 40: 1375-1381
- Microbiome of deep dentinal caries lesions in teeth with symptomatic irreversible pulpitis.PLoS One. 2016; 11: e0154653
Published online: February 22, 2023
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