Abstract
Introduction
The aim of this study was to assess whether the biological characteristics of dental
pulp stem cells (DPSCs), such as viability, adhesion to dentin, mineralization, and
release of immunomodulatory cytokines, are affected by the inflammatory status of
the donor tissue and/or the sustained inflammatory environment.
Methods
DPSCs were isolated from pulps from 3 caries-free teeth (healthy or hDPSCs), and from
3 teeth with irreversible pulpitis or deep caries (unhealthy DPSCs or uDPSCs). The
cells were cultured in odontogenic and osteogenic media with or without lipopolysaccharides.
Viability was analyzed by MTT assay at days 1, 3, 5, and 7; adhesion to dentin was
evaluated through an environmental scanning electron microscope after 48 hours and
through MTT assay; mineralization was analyzed with alizarin red staining after 21 days;
and the release of proinflammatory (interleukin 6) and immunosuppressive cytokines
(interleukin 10) was measured with the enzyme-linked immunosorbent assay after 24 hours
and 7 days.
Results
The inflammatory status of the pulp significantly reduced the viability and mineralization
capacity of the DPSCs, although it did not affect the adhesion capacity to dentin
or the secretion of the proinflammatory interleukin. The inflammatory microenvironment
(lipopolysaccharide) only had a significant impact on the secretion of interleukin
6, which was augmented after 7 days.
Conclusions
The inflammatory status of the dental pulp should be taken into account when the use
of DPSCs is intended either for research and/or for application in reparative or regenerative
therapies.
Key Words
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Article info
Publication history
Published online: February 22, 2023
Identification
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© 2023 American Association of Endodontists.
